1,386 research outputs found
Comparative Analysis of the Major Polypeptides from Liver Gap Junctions and Lens Fiber Junctions
Gap junctions from rat liver and fiber junctions from bovine lens have similar septilaminar profiles when examined by thin-section electron microscopy and differ only slightly with respect to the packing of intramembrane particles in freeze-fracture images. These similarities have often led to lens fiber junctions being referred to as gap junctions. Junctions from both sources were isolated as enriched subcellular fractions and their major polypeptide components compared biochemically and immunochemically. The major liver gap junction polypeptide has an apparent molecular weight of 27,000, while a 25,000-dalton polypeptide is the major component of lens fiber junctions. The two polypeptides are not homologous when compared by partial peptide mapping in SDS. In addition, there is not detectable antigenic similarity between the two polypeptides by immunochemical criteria using antibodies to the 25,000-dalton lens fiber junction polypeptide. Thus, in spite of the ultrastructural similarities, the gap junction and the lens fiber junction are comprised of distinctly different polypeptides, suggesting that the lens fiber junction contains a unique gene product and potentially different physiological properties
Correlation between stick-slip frictional sliding and charge transfer
A decade ago, Budakian and Putterman (Phys. Rev. Lett., {\bf 85}, 1000
(2000)) ascribed friction to the formation of bonds arising from contact
charging when a gold tip of a surface force apparatus was dragged on
polymethylmethacrylate surface. We propose a stick-slip model that captures the
observed correlation between stick-slip events and charge transfer, and the
lack of dependence of the scale factor connecting the force jumps and charge
transfer on normal load. Here, stick-slip dynamics arises as a competition
between the visco-elastic and plastic deformation time scales and that due to
the pull speed with contact charging playing a minor role. Our model provides
an alternate basis for explaining most experimental results without ascribing
friction to contact charging.Comment: 8 pages, 4 figures, To be appeared in Physical Review
Attractive Casimir Forces in a Closed Geometry
We study the Casimir force acting on a conducting piston with arbitrary cross
section. We find the exact solution for a rectangular cross section and the
first three terms in the asymptotic expansion for small height to width ratio
when the cross section is arbitrary. Though weakened by the presence of the
walls, the Casimir force turns out to be always attractive. Claims of repulsive
Casimir forces for related configurations, like the cube, are invalidated by
cutoff dependence.Comment: An updated version to coincide with the one published December 2005
in PRL. 4 pages, 2 figure
Warm tachyonic inflation in warped background
We analyze warm tachyonic inflation, proposed in the literature, but from the
viewpoint of four dimensional effective action for tachyon field on a non-BPS
D3-brane. We find that consistency with observational data on density
perturbation and validity of effective action requires warped compactification.
The number of background branes which source the flux is found to be of the
order of 10 in contrast to the order of in the standard cold
inflationary scenario.Comment: 9 pages, RevTe
Magnetic field generation in Higgs inflation model
We study the generation of magnetic field in Higgs-inflation models where the
Standard Model Higgs boson has a large coupling to the Ricci scalar. We couple
the Higgs field to the Electromagnetic fields via a non- renormalizable
dimension six operator suppressed by the Planck scale in the Jordan frame. We
show that during Higgs inflation magnetic fields with present value
Gauss and comoving coherence length of can be generated in the
Einstein frame. The problem of large back-reaction which is generic in the
usual inflation models of magneto-genesis is avoided as the back-reaction is
suppressed by the large Higgs-curvature coupling.Comment: 10 pages, RevTeX
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Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32
Background
The gap junction protein, Connexin32 (Cx32), is expressed in various tissues including liver, exocrine pancreas, gastrointestinal epithelium, and the glia of the central and peripheral nervous system. Gap junction-mediated cell-cell communication and channel-independent processes of Cx32 contribute to the regulation of physiological and cellular activities such as glial differentiation, survival, and proliferation; maintenance of the hepatic epithelium; and axonal myelination. Mutations in Cx32 cause X-linked Charcot–Marie–Tooth disease (CMT1X), an inherited peripheral neuropathy. Several CMT1X causing mutations are found in the cytoplasmic domains of Cx32, a region implicated in the regulation of gap junction assembly, turnover and function. Here we investigate the roles of acetylation and ubiquitination in the C-terminus on Cx32 protein function. Cx32 protein turnover, ubiquitination, and response to deacetylase inhibitors were determined for wild-type and C-terminus lysine mutants using transiently transfected Neuro2A (N2a) cells.
Results
We report here that Cx32 is acetylated in transfected N2a cells and that inhibition of the histone deacetylase, HDAC6, results in an accumulation of Cx32. We identified five lysine acetylation targets in the C-terminus. Mutational analysis demonstrates that these lysines are involved in the regulation of Cx32 ubiquitination and turnover. While these lysines are not required for functional Cx32 mediated cell-cell communication, BrdU incorporation studies demonstrate that their relative acetylation state plays a channel-independent role in Cx32-mediated control of cell proliferation.
Conclusion
Taken together these results highlight the role of post translational modifications and lysines in the C-terminal tail of Cx32 in the fine-tuning of Cx32 protein stability and channel-independent functions
Solid State Systems for Electron Electric Dipole Moment and other Fundamental Measurements
In 1968, F.L. Shapiro published the suggestion that one could search for an
electron EDM by applying a strong electric field to a substance that has an
unpaired electron spin; at low temperature, the EDM interaction would lead to a
net sample magnetization that can be detected with a SQUID magnetometer. One
experimental EDM search based on this technique was published, and for a number
of reasons including high sample conductivity, high operating temperature, and
limited SQUID technology, the result was not particularly sensitive compared to
other experiments in the late 1970's.
Advances in SQUID and conventional magnetometery had led us to reconsider
this type of experiment, which can be extended to searches and tests other than
EDMs (e.g., test of Lorentz invariance). In addition, the complementary
measurement of an EDM-induced sample electric polarization due to application
of a magnetic field to a paramagnetic sample might be effective using modern
ultrasensitive charge measurement techniques. A possible paramagnetic material
is Gd-substituted YIG which has very low conductivity and a net enhancement
(atomic enhancement times crystal screening) of order unity. Use of a
reasonable volume (100's of cc) sample of this material at 50 mK and 10 kV/cm
might yield an electron EDM sensitivity of e cm or better, a factor
of improvement over current experimental limits.Comment: 6 pages. Prepared for ITAMP workshop on fundamental physics that was
to be held Sept 20-22 2001 in Cambride, MA, but was canceled due to terrorist
attack on U.S New version incorporates a number of small changes, most
notably the scaling of the sensitivity of the Faraday magnetometer with
linewidth is now treated in a saner fashion. The possibility of operating at
an even lower temperarture, say 10 microkelvin, is also discusse
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